Synonyms

Obese Protein, Obesity Factor

Accession

P41159

GeneID

3952

Source

E.coli-derived Human Leptin, Val22-Cys167.

Molecular Weight

Approximately 16.0 kDa.

Tag

None

Purity

> 95% by SDS-PAGE.

Biological Activity

Immobilized Human Leptin Antibody at 3 μg/mL (100 μL/well) can bind Human Leptin , the EC₅₀ of Human Leptin is 174-246 pg/mL.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1%BSA.

Reconstitution

1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.     Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum.