SwissProt ID

P68431

基因ID

8290

免疫原

Purified recombinant protein fragments expressed in E.coli.

緩沖液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

分子量

Observed MW (kDa):15

克隆類型

Monoclonal Antibody

亞型

IgG1

宿主

Mouse

物種反應

Human,Mouse,Rat,Yeast

純化方式

Affinity Purified

細胞定位

Chromosome,Nucleus

研究領域

-

IF analysis of Hela with DAPI (Left) and (Right) diluted at 1:200

1 、 Input: Hela Cell Lysate 2 、 IP product: IP dilute 1:20 Western blot analysis: primary

antibody : 1:2000,. Secondary antibody: Goat anti-Mouse IgG(H+L) (S1) 1:10000

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H3 Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tem- peRature, 30min). Negative control was used by secondary antibody only.

Western blot detection of Histone H3 in C6,3T3,Hela cell lysates using Histone H3 Mouse mAb(1:10000 diluted).Predicted band size:15KDa.Observed band size:15KDa. 

Immunoprecipitation analysis of Hela cell lysates using Histone H3 Mouse mAb