表達區(qū)間及表達系統(tǒng)（Source）

Cynomolgus/Rhesus macaque CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ala20-Arg191.[Accession | XP_005569963.1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Cynomolgus CD27 Ligand, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 70.8ng/ml determined by ELISA. 

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.