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  煙雨笑春風 2019-04-19 17:07:04

  <p><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">用臺盼藍拒染法計數活細胞:</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">1. 徹底混合細胞懸液,取100礚樣本至一支試管中.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">2. 臺盼藍染色有兩種方法.一是首先用細胞培養液稀釋細胞樣本,然后用臺盼藍溶液(產品號 #07050) 1/2稀釋樣本（細胞和臺盼藍的稀釋比為1:1）.或者直接用臺盼藍溶液稀釋樣本.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">例如：1/40稀釋樣本</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">加入50礚 細胞懸液至950礚 IMDM+2% FBS (1/20 稀釋),混合,然后取100礚稀釋的細胞懸液加入100礚臺盼藍溶液中(終稀釋度為1/40).或取20礚細胞懸液加入780礚臺盼藍溶液中(終稀釋度為1/40).</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">3. 渦懸振蕩,混合稀釋的細胞樣本.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">4. 血細胞計數器的準備：首先用酒精清潔其小室和蓋玻片,然后用脫脂綿擦干.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">5. 仔細的將蓋玻片覆蓋兩個小室.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">6. 用微量移液器或毛細管吸取稀釋的樣本.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">7. 用微量移液器或毛細管將樣本充滿兩個小室.不要過滿或不足.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">8. 計數4個大方格中細胞核的總數(1x1x0.1mm)或&gt;100個細胞.分別死細胞和活細胞.死細胞染成藍色的死細胞(因為細胞完整性破壞,細胞吸收臺盼藍),活細胞透明有折光（未染色）.</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">9. 活細胞計數按以下方法計算：</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 20px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋體, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="17_6">每個方格中活細胞總數平均值x稀釋倍數x104= 活細胞數/mL</span></p>

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