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浮游植物分類熒光儀——PHYTO-PAM-II

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詳細介紹

主要功能

●對自然水體中的藍藻、綠藻、硅/甲藻和隱藻自動分類(定性)

●自動測量水樣中藍藻、綠藻、硅/甲藻和隱藻的葉綠素 a 含量(定量)和總葉綠素 a 含量

●一杯自然水樣,同時獲得藍藻、綠藻、硅/甲藻和隱藻的光合活性:

●光合效率和光合速率(相對電子傳遞速率)

●快速光曲線并進行擬合

●藻類的潛在ZD光合效率

●藻類的光保護能力

●藻類耐受強光的能力

●用戶可做自己的參考光譜


測量參數

Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和葉綠素含量等

應用領域

●主要用于水生生物學、水域生態學、海洋學、湖沼學、水質預警、微藻生理學、微藻抗逆性等領域,對于了解自然水樣中藻類種群的動態變化、水華預警、野外水體中光合作用的時空變化、校正初級生產力的計算等有較大幫助。

●特別適于浮游植物動力學研究和有害水華的早期預警。

主要技術參數

●主控單元:金屬外殼,包含所有的光電元件及樣品測量室

●測量光: LED,440 nm,480 nm,540 nm,590 nm 和 625 nm,5 波長脈沖調制測量光,2 檔強度設置,8 檔調制頻率設置, 3 檔測量光自動高頻設置

●光化光:板載多波長 LED 陣列,440 nm,480 nm,540 nm,590 nm,625 nm 和 420-640 nm(白光),提供持續光化光,ZG可達 1400 μmol m-2 s-1,快速動力學閃光高達 7000 μmol m-2 s-1,飽和脈沖ZG可達 5000 μmol m-2 s-1

●遠紅光:725 nm LED

●信號檢測:基于 H-10720 光電傳感器模塊的光電倍增檢測器

●標準檢測過濾器:> 650 nm 長通濾光片

●高分辨率的光電倍增管,葉綠素濃度檢測限低至 0.1μg/L,適用野外采集的藻濃度很稀的樣品

升級的技術特點

●可提供 5 種波長的脈沖調制測量光和光化光

●板載 LED 陣列芯片技術

●可實時進行四種藻的分類

●可進行標準 PAM 測量及不同波長強光化光誘導的毫秒級熒光上升動力學分析

●可測定光系統 II 功能性捕光截面積

●內置自動測量程序,易于操作

PHYTO-PAM-II vs PHYTO-PAM

便攜式 PHYTO-PAM-II

PHYTO-PAM

5 種不同波長的測量光用于生物體內不同類型的天線色素熒光的激發

4 種不同波長的光

4 種色素類型的在線分類

3 種色素類型的在線分類

6 種波長的光化光

1 種波長的光化光

可以分別測量綠藻,藍藻,硅/甲藻以及含有藻紅蛋白的有機體,如隱藻不同波長下 PSII 的活性

無該功能

緊湊型設計

需要組裝

增加了快速動力學操作模式,可通過強光化光脈沖,測量不同波長的 O- I1 熒光上升動力學曲線

無該功能

通過測定不同光質和光合生物色素復合體,獲得光系統 II 功能性捕光截面積的信息即 σPSII

無該功能

通過 FluoRed 熒光標準將參考光譜校準標準化

無該功能

獲得的參考光譜可在不同設備及用戶間互換使用

參考光譜不能互換

選購指南

一、實驗室測量基本款

系統組成:實驗室版主機,激發檢測單元,懸浮液的光學單元,球形光量子傳感器,工作臺,軟件等

注意:新版 PHYTO-PAM-II 必須配置光量子傳感器,校準光強后參考光譜可在不同儀器間通用

Phyto-PAM II laboratory version.jpg

實驗室測量基本款

二、野外便攜緊湊款

系統組成:緊湊型主機,球形光量子傳感器,軟件等

注意:新版 PHYTO-PAM-II 必須配置光量子傳感器,校準光強后參考光譜可在不同儀器間通用

Phytho-PAMM-II_071_1-small-2.jpg

便攜式緊湊款


Channels.jpg

algae.jpg

Reference.jpg

五種測量光通道

區分四種藻類

通用型參考光譜

軟件界面7.png

light-curve.jpg

Fast-Kinetics.jpg

測量慢速動力學曲線

淬滅分析,暗弛豫分析

測量快速光曲線

光響應曲線

測量快速動力學曲線

分析PSII功能性捕光截面


三、其他可選附件

1,WATER-S:攪拌器(推薦選配),利用內置電池驅動的馬達帶動攪拌棒旋轉,對樣品杯里的懸浮液進行攪拌。需配置攪拌棒 WATER-R。

2,WATER-R:攪拌棒(標配10根)

產地:德國WALZ


參考文獻

數據來源:光合作用文獻 Endnote 數據庫,更新至 2021 年 1 月,文獻數量超過 10000 篇

原始數據來源:Google Scholar

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